Overview
A protocol for full-length polyadenylated RNA
- This protocol is for full-length polyadenylated tail RNA
- Starting material can be total RNA or an enriched sample (e.g. poly(A)+ or ribodepleted)
- Native RNA is sequenced
- No fragmentation
- 2 hr library prep
For Research Use Only
Introduction to the protocol
Library preparation
Sequencing and data analysis
Troubleshooting