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Barcoding overview
A user can barcode their data directly in MinKNOW after a sequencing experiment has finished, using FASTQ data.
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Select the input folder containing the FASTQ data to barcode from a previous run.
Select whether you want to process FASTQ reads in sub-directories.
If your chosen read input folder contains sub-directories with FASTQ files, you can choose whether to barcode FASTQ files in these sub-directories by switching this option on.
Note: Data can only be saved to the
/data
folder. -
Choose output folder for the post-run barcoded data.
By default, MinKNOW will create a
/barcoding
folder in/data/
. You can set a different folder which to save the barcoded reads.
Note: This must be a sub-folder of/data
directory. -
Click 'Start' to begin post-run barcoding.