- Materials
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- Flow Cell Wash Kit (EXP-WSH004) or Flow Cell Wash Kit XL (EXP-WSH004-XL)
- Flow cell priming reagents available in your sequencing kit or in the following kits:
- Sequencing Auxiliary Vials V14 (EXP-AUX003)
- Flow Cell Priming Kit (EXP-FLP004)
- Equipment
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- P1000 pipette and tips
- P20 pipette and tips
- Ice bucket with ice
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Reloading a library
Additional buffers are required for reloading a library following the washing of a flow cell. These can be found in the one of the following expansion kits:
Sequencing Auxiliary Vials V14 (EXP-AUX003). This expansion contains vials of Sequencing Buffer (SB), Elution Buffer (EB), Library Solution (LIS), and Library Beads (LIB) with Kit 14 flow cell priming reagents: Flow Cell Flush (FCF) and Flow Cell Tether (FCT). This expansion is only compatible with our Kit 14 chemistry e.g. SQK-LSK114.
Flow Cell Priming Kit (EXP-FLP004). This expansion contains both Kit 14 flow cell priming reagents required: Flow Cell Flush (FCF) and Flow Cell Tether (FCT). This expansion is only compatible with Kit 14 chemistry.
For our previous chemistries:
Sequencing Auxiliary Vials expansion (EXP-AUX001). This expansion contains vials of Elution Buffer (EB), Sequencing Buffer (SQB), and Loading Beads (LB), additional to those found in DNA sequencing kits for our Kit 9 chemistry.
Sequencing Auxiliary Vials expansion (EXP-AUX002). This expansion contains vials of Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS), and Loading Beads II (LBII), additional to those found in Kit 10 and 11 chemistry, such as:
- Kit 10 e.g. SQK-LSK110
- Kit 11 e.g. SQK-PCS111
- Flow Cell Priming Kit (EXP-FLO002). This expansion contains both flow cell priming reagents required: Flush Buffer (FB) and Flush Tether (FLT). This expansion is compatible with Kit 9, 10 and 11 chemistry.
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Flow Cell Wash Kit (EXP-WSH004) contents
Contents Volume (µl) No. of tubes No. of uses Wash Mix (WMX) 15 1 6 Wash Diluent (DIL) 1,300 2 6 Storage Buffer (S) 1,600 2 6 - Wash Mix (WMX) contains DNase I.
- Wash Diluent (DIL) contains the exonuclease buffer that maximises activity of the DNase I.
- The Storage Buffer allows flow cells to be stored for extended periods of time.
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Flow Cell Wash Kit XL (EXP-WSH004-XL) contents
Name Acronym Fill volume per vial (µl) Cap colour No. of vials No. of uses Wash Mix WMX 150 Brown 1 48 Wash Diluent DIL 20,000 White cap, brown stripe 1 48 Storage Buffer S 25,000 White cap, yellow stripe 1 48 - Wash Mix (WMX) contains DNase I.
- Wash Diluent (DIL) contains the exonuclease buffer that maximises activity of the DNase I.
- The Storage Buffer allows flow cells to be stored for extended periods of time.
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Flow Cell Priming Kit (EXP-FLP004) contents
Name Acronym Cap colour No. of vials Fill volume per vial (μl) Flow Cell Flush FCF 6 Clear cap, light blue lable 8,000 Flow Cell Tether FCT 1 Purple 200 -
Sequencing Auxiliary Vials V14 (EXP-AUX003) contents
Name Acronym Cap colour No. of vials Fill volume per vial (μl) Elution Buffer EB Black 2 500 Sequencing Buffer SB Red 2 700 Library Solution LIS White cap, pink label 2 600 Library Beads LIB Pink 2 600 Flow Cell Flush FCF Light blue label 2 8,000 Flow Cell Tether FCT Purple 2 200