- Materials
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- Flow Cell Wash Kit (EXP-WSH004) or Flow Cell Wash Kit XL (EXP-WSH004-XL)
- Consumables
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- P1000 pipette and tips
- P20 pipette tips
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Storage Buffer (S) can be used to flush flow cells for storage for later use or to check number of available nanopores before loading another library.
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Thaw one tube of Storage Buffer (S) at room temperature.
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Mix contents thoroughly by pipetting and spin down briefly.
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Slide the inlet port cover clockwise to open the inlet port.
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After opening the inlet port, check for a small air bubble under the cover. Draw back a small volume to remove any bubbles:
- Set a P1000 pipette to 200 µl
- Insert the tip into the inlet port
- Turn the wheel until the dial shows 220-230 µl, or until you can see a small volume of buffer entering the pipette tip.
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Slowly add 500 μl of Storage Buffer through the inlet port of the flow cell.
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Close the inlet port cover and remove any buffer from the waste port.
The waste liquid can be aspirated from either one of the ports, labelled 2 and 3 on the flow cell.
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The flow cell can now be stored at 4-8°C.