- Materials
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- Rapid Adapter (RA)
- Adapter Buffer (ADB)
- Elution Buffer (EB)
- Consumables
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- 1.5 ml Eppendorf DNA LoBind tubes
- Equipment
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- Microfuge
- Ice bucket with ice
- P1000 pipette and tips
- P200 pipette and tips
- P100 pipette and tips
- P20 pipette and tips
- P10 pipette and tips
- P2 pipette and tips
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Thaw the kit components at room temperature, spin down briefly using a microfuge and mix by pipetting as indicated by the table below:
Reagent 1. Thaw at room temperature 2. Briefly spin down 3. Mix well by pipetting Rapid Adapter (RA) Not frozen ✓ ✓ Adapter Buffer (ADB) Not frozen ✓ ✓ -
In a fresh 1.5 ml Eppendorf DNA LoBind tube, dilute the Rapid Adapter (RA) as follows and pipette mix:
Reagents Volume Rapid Adapter (RA) 1.5 μl Adapter Buffer (ADB) 3.5 μl Total 5 μl -
Add 1 μl of the diluted Rapid Adapter (RA) to the amplified cDNA library, making the total volume 12 μl.
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Mix gently by flicking the tube, and spin down.
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Incubate the reaction for 5 minutes at room temperature.
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Spin down briefly.