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Discover nanopore sequencing

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Discover nanopore sequencing

What can it do? How does it work? Our platform performance and accuracy

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Prepare Sequence Analyse
Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Research areas

Investigations

Techniques

Research areas

Microbiology Human genetics Microbiome Clinical research Cancer Plant Transcriptome Animal Population genomics COVID 19 Environmental research & conservation Infectious disease

Investigations

Structural variation SNVs and phasing Gene expression Identification Splice variation Assembly Fusion transcripts Epigenetics Single cell Chromatin conformation

Techniques

Whole genome Targeted RNA & cDNA sequencing Metagenomics Short fragment mode
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Documentation Prepare Library Prep Protocols

Rapid sequencing gDNA - barcoding (SQK-RBK004)

Start Protocol Download Checklist Download PDF Previous versions

Overview

The fastest and simplest protocol for genomic DNA involving:

  • 10 mins library prep
  • high yield
  • fragmentation

For Research Use Only

This is a Legacy product
This kit has now been discontinued and we recommend all customers upgrade to the latest chemistry for their relevant kit which is available on the Store.

Introduction to the protocol

Overview of the protocol

Equipment and consumables

Library preparation ~20 minutes

Library preparation

Priming and loading the SpotON Flow Cell

Sequencing and data analysis

Data acquisition and basecalling

Downstream analysis

Ending the experiment

Troubleshooting

Issues during DNA/RNA extraction and library preparation

Issues during the sequencing run

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