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Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Documentation Rapid sequencing DNA V14 - barcoding (SQK-RBK114.24 or SQK-RBK114.96)

Rapid sequencing DNA V14 - barcoding (SQK-RBK114.24 or SQK-RBK114.96)

Start Protocol Download Checklist Download PDF Previous versions

Overview

  • This protocol uses genomic DNA
  • For multiplexing 1-96 samples
  • Library preparation time ~60 minutes
  • High yield
  • Fragmentation
  • Compatible with R10.4.1 flow cells

For Research Use Only

This is an Early Access product
For more information about our Early Access programmes, please see this article on product release phases.

Introduction to the protocol

Overview of the protocol

Equipment and consumables

Computer requirements and software

Library preparation ~70 minutes

Library preparation

Priming and loading the SpotON flow cell

Sequencing and data analysis

Data acquisition and basecalling

Downstream analysis

Ending the experiment

Troubleshooting

Issues during DNA/RNA extraction and library preparation

Issues during the sequencing run using a Rapid-based sequencing kit

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