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Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Microbiology Human genetics Microbiome Clinical research Cancer Plant Transcriptome Animal Population genomics COVID-19 Environmental research & conservation Infectious disease

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Structural variation SNVs and phasing Gene expression Identification Splice variation Assembly Fusion transcripts Epigenetics Single cell Chromatin conformation

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Whole genome Targeted Whole transcriptome Metagenomics Short fragment mode View all Applications
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Documentation Prepare Library Prep Protocols

Direct RNA sequencing - sequence-specific (SQK-RNA002)

Start Protocol Download Checklist Download PDF Previous versions

Overview

A protocol for full-length RNA

  • Select RNA targets of your choice
  • Native RNA is sequenced
  • No fragmentation
  • 2 h library prep

For Research Use Only

Introduction to the protocol

Overview of the protocol

Equipment and consumables

Computer requirements and software

Library preparation ~130 minutes

Library preparation

Priming and loading the SpotON flow cell

Sequencing and data analysis

Data acquisition and basecalling

Downstream analysis

Ending the experiment

Troubleshooting

Issues during DNA/RNA extraction and library preparation

Issues during the sequencing run

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