- Consumables
-
- 1.5 ml Eppendorf DNA LoBind tubes
- Equipment
-
- P1000 pipette and tips
- P100 pipette and tips
- P200 pipette and tips
- P20 pipette and tips
- Wide-bore pipette tips
-
Day 3: Ultra-long DNA experiment overview
The eluate from the overnight incubation is removed and stored on ice until it is ready for loading onto the PromethION Flow Cell.
-
Gently mix the DNA library by slowly pipetting 10 times with a wide-bore pipette tip.
Thorough but gentle resuspension of DNA is required to prevent heterogeneity in the sample.