8th December 2023
- Removed references to Guppy throughout the document
19th September 2023
- Added a new page (Sequencing high output simplex data) to the introduction to explain how to calculate pore occupancy
28 June 2023
- Add information for ensure the best basecalling performance when using Dorado in "Basecalling Kit 14 duplex data".
May 2023
- Removed references to altering basecalling speed to be aligned with the new MinKNOW software across the protocol: software release 23.04.X
5th Oct 2022
- corrected the config file used in 'Basecalling Kit 14 simplex data' and 'Basecalling kit 14 duplex data'
4th Oct 2022
- Updated the title from Kit 14 device and informatics to 'Kit 14 sequencing and duplex basecalling'
- Reformatted the protocol with new sections introducing Kit 14 chemistry and duplex basecalling
- Simplex basecalling sections have been combined for simplex basecalling on MinKNOW and guppy
- Instructions for basecalling in MinKNOW has been updated for new MinKNOW 22.08.9 release
- Instructions for duplex have been updated and improved
- The introduction to read splitting and assembly have been moved into the Appendix
28th Sept 2022
- Updated to clarify kit 14 basecalling is available for all V14 kits currently release and across our MinION, GridION and PromethION devices
7th September 2022
Update relevant for the MinKNOW 22.08 release:
- The Running Kit 14 chemistry on your device" section has been updated to remove the need to download configuration files.
10th August 2022
- Removed reference to release just running at 400 bps to align with updates
29th July 2022
- Updated 'Basecalling Kit 14 simplex data in MinKNOW' to clarify either Linux or Windows operating systems may be used to basecall.