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Nanopore Learning / Courses /
Control Experiment: Rapid sequencing guide

This course provides guidance for running a control experiment on a MinION using the Rapid Sequencing Kit

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Course overview

Planning

1 DNA (Lambda) Control Experiment Overview
2 MinION introduction
3 Flow cell introduction
4 Flow cell handling

Preparation

5 MinKNOW: Introduction
6 EPI2ME Introduction
7 MinKNOW: Installation
8 EPI2ME Installation
9 MinKNOW: Hardware check
10 MinKNOW: Flow cell check

Sequencing

11 Rapid Lambda Control Experiment
12 Library preparation: Rapid sequencing kits
13 Priming and loading your flow cell
14 MinKNOW: Setting up a new experiment

Analysis

15 Lambda Control Experiment Run Metrics and Results
Course completion

Library preparation: Rapid sequencing kits

6 min
Learning objectives
  • Core concepts of Nanopore sequencing
  • Factors essential in making a molecule suitable for Nanopore sequencing will be identified
  • The differences between the rapid versus the ligation sequencing kit
  • How input requirement and the relationship to sequencing throughput
  • The multiplexing compatibilities for this sequencing kit
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