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Nanopore Learning / Lesson library /
Knowledge Exchange: cDNA sequencing on the Oxford Nanopore platform

This Knowledge Exchange introduces cDNA sequencing with Oxford Nanopore technology - from library prep to analysis.

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Learning objectives
  • Understand how each cDNA sequencing kit works, and which one to use for each application
  • Learn some tips and tricks at the bench, for sample and library prep
  • Learn about the analysis algorithm Pychopper
  • Learn about the isoform reconstruction tool Pinfish
  • Learn how to examine differential gene expression and transcript usage
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