February 2023
- Updated product phase to 'Legacy'
1st August 2022
- Removed references to FFPE in the End-prep step.
- Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
14th July 2022
- updated compabilities and removed references to discontinued flow cells
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
26th January 2022
- Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
October 2021
- Clarified R10.3 flow cell loading recommendations
14th Sept 2021
- In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
13th Sept 2021
- Added tables outlining kit contents to the Equipment and Consumables section.
21st April 2021
Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions:
- EXP-AMII001 is included in the "Equipment and consumables" section
- Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
11th Jan
Updated mastermix volumes to volumes required per sample
4th Jan
Equipment list updated
8th Dec
Compatibilities updated to EXP-WSH004
9th Nov
Updated equipment and consumables
18th May
Updated with corrections to RT step and Native Barcode Ligation
18th May
Updated with corrections to RT step and Native Barcode Ligation