February 2023
- Updated legacy wording regarding kit availability in the "Overview of the protocol".
January 2023
- Update the priming and loading the flow cells for PromethION with new diagrams.
27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
- Updated product phase to Legacy.
20th June 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
24th Febrary 2022
Updated EXP-AUX110 to new name EXP-AUX002
27th January 2022
Updated the "Mixing method" to include pipette mixing 10-20 times for component combination in all ligation reactions, DNA repar and end-prep.
In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
Download pdf for Minion Download pdf for Promethion Download pdf for Gridion14th January 2022
In "Priming and loading the flow cell", the first "Important" note has been updated to say that the Kit 12 chemistry now runs at 30°C on all nanopore sequencing devices.