19th September 2023
- Updated flow cell loading instructions to include light shield installation
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
13th Sept 2021
- Added table of contents in the Equipments and Consumables section.
9th Dec
Compatibilities updated for EXP-WSH004
3rd Dec 2020
Updated the "Notes on multiple crRNAs" in the Preparing the Cas9 ribonucleoprotein complexes (RNPs) step to say:
If you have validated a set of crRNA probes that are always run together, we recommend pooling all crRNA probes and then aliquoting that pool and freezing each aliquot at -80°C. Each time you run a Cas9 experiment and need to make an RNP complex, take out a crRNA pool aliquot and combine with the tracrRNA.