12th July 2023
- Updated product legacy availability
January 2023
- Changed the PromethION "Priming and loading the flow cell" section to the updated version.
11th November
- Product status updated to Legacy
25th October 2022
- Added link to newest Kit 11 protocol version
July 2022
- Updated the 'Selecting for full-length transcripts by PCR' to include another mixing step and increase the amount of ethanol required.
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
13th Sept 2021
- Added table outlining kit content in the Equipment and Consumables section.
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
9th Dec
Compatibilities updated for EXP-WSH004
8th October 2019
Updated guidelines for the optimum amount of cDNA library to load into the flow cell: the new recommendation is 100 fmols.
27th September 2019
Updated and expanded Troubleshooting guide
23rd August 2019
Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
19th July 2019
Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
Updated with new information for MinKNOW release v19.06:
- Compression of queued reads
- Pause functionality
- UI improvements including:
- Flow cell health at platform QC
- Flow cell status on landing page
- R10 flow cell running scripts
- New Fast Models for RNA and DNA
- PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files
- Flongle hardware check for confirming Flongle adapter functions correctly
Updated for use with EXP-FLP002, retaining instructions for EXP-FLP001.PRO.6
Download pdfUpdates for MinKNOW release 19.05
Download pdfCorrection to the flow cell priming and loading section of the protocol
Download pdf