May 2023
- Updated kit availability
February 2023
- Updated product phase to 'Legacy'
January 2023
- Changed the PromethION "Priming and loading the flow cell" section to the updated version.
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
13th Sept 2021
- Added table outlining kit contents in the Equipment and Consumables section.
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
22nd December 2020
Adapter Bead Binding Buffer (ABB) has been substituted in the kit for Wash Buffer (WSB); the kit contents and protocol instructions for the "AMPure XP Bead Binding" step have changed to reflect this.
9th Dec
Compatibilities updated for EXP-WSH004
24th December 2019
Fixing erroneous instructions
27th September 2019
Updated and expanded Troubleshooting guide
23rd August 2019
Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
19th July 2019
Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
Updated with new information for MinKNOW release v19.06:
- Compression of queued reads
- Pause functionality
- UI improvements including:
- Flow cell health at platform QC
- Flow cell status on landing page
- R10 flow cell running scripts
- New Fast Models for RNA and DNA
- PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files
- Flongle hardware check for confirming Flongle adapter functions correctly
Updated for use with EXP-FLP002, retaining instructions for EXP-FLP001.PRO.6
Download pdfUpdates for MinKNOW release 19.05
Download pdfCorrection to the flow cell priming and loading section of the protocol
Download pdf