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Flow cell check
We strongly recommend performing a flow cell check before loading a DNA or RNA library to assess the number of pores available.
Oxford Nanopore Technologies will replace any flow cell that falls below the warranty number of active pores within three months of purchase, provided that you report the results within two days of performing the flow cell check and you have followed the storage recommendations.
Flow cell Minimum number of active pores covered by warranty Flongle Flow Cell (FLO-FLG001) 50 MinION/GridION Flow Cell 800 PromethION Flow Cell 5000 -
When you see the flow cell type and the flow cell ID is recognised, click 'Start' to begin.
This example is on a GridION device:
Note: For Flongle, the flow cell ID must be filled in manually.
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Flow cell health indicators
The quality of the flow cell will be shown as one of the three outcomes on the Sequencing Overview page:
Yellow exclamation mark: The number of sequencing pores is below warranty.
Green tick: The number of sequencing pores is above warranty and ready for sequencing.
Question mark: A flow cell check has not been run on the flow cell during this MinKNOW session.
Note: the indicator of quality will only remain visible during the MinKNOW session when testing occurred. Once the sequencing device has been re-started, the status of the flow cell will be erased.