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The MinION Mk1C
This device combines the real-time, rapid, portable sequencing of a MinION Mk1B with a Graphical Processing Unit (GPU) and a high-resolution screen. It can be used with MinION Flow Cells, as well as the Flongle Adapter and flow cells. The device is operated by the MinKNOW software that controls the device, experimental scripts, and performs basecalling.
Below is a quick start guide to set up a new device and to start sequencing your library samples. For further details using this device, please see the MinION Mk1C User Manual.
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Set up the MinION Mk1C device.
Connect the AC/DC adapter to a power source and connect the MinION Mk1C.
Switch the power source on and press the power button on the device.
For the first time, users will be taken through the onboarding process to set up the device. Through this process, you will:
- Connect your device to the internet (Ethernet recommended)
- Log into your MinKNOW account using your Community credentials
- Configure local network access to your device
- Perform a hardware check
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Check the time and date of your device.
Verify the time and data of your device is correct to successfully establish a network connection.
Navigate to Device settings in the host settings.
Check the time and date.
Click Change data and time and set the time using custom date/time.
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Reset the default password.
The device is shipped with a default password
minit
that must be reset.Navigate to Device settings in the host settings.
Click Reset password to open the Change password dialogue box.
Enter the current password, followed by the new password.
Click Reset password to confirm the password change.
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Tutorials
These are available to help you get started and run your experiments as easily as possible.
On opening the MinKNOW software for the first time, the tutorials will navigate you through the graphical user interface (GUI). Click Continue tutorial to follow or click the ⁞ to skip.
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Perform a hardware check.
A hardware check must be performed on new devices or when software has been updated.
Open the MinION Mk1C and slide the CTC under the clip. Gently press down onto the CTC until it clicks into place. To check a Flongle Adapter, insert an empty adapter instead of a CTC.
Close the device lid.
Navigate to the Start page and click Hardware check.
Click Start and the hardware check will complete after approximately 1 minute.
After the hardware check has finished, remove the CTCs and store in dry conditions.
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Perform a flow cell check.
A flow cell check must be carried out before loading a DNA or RNA library to assess the number of pores available.
Open the device and slide your flow cell under the clip. Firmly press down on the flow cell. For Flongle, slide the Flongle Adapter under the clip and insert a Flongle Flow Cell.
Navigate to the Start page and click Flow Cell Check.
MinKNOW will recognise the MinION Flow Cell type and IDs.
Note: For Flongle, fill in the flow cell ID manually.Click Start and the flow cell check will begin.
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Set up a sequencing run.
Prime and load your flow cell with DNA or RNA library by following a library prep protocol and set up a sequencing run, as follows:
Navigate to the Start page and click Start Sequencing.
In the Positions tab, fill in the experiment name, sample ID, and select the flow cell type. Click Continue to kit selection.
Note: For Flongle, fill in the flow cell ID manually.Select the kit and any expansion(s) used to prepare the library. Click Continue to run options.
Specify the sequencing run length and minimum read length or keep the default settings. Click Continue to analysis.
Choose your basecaller model and select any available barcoding and alignment options or keep the default settings. Click Continue to output.
Specify your output data location, format and filtering options or keep the default settings. Click Continue to final review.
Click Start.
For further details, please see the Starting a sequencing run section for your device in the MinKNOW protocol.