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Sequencing run template
Sequencing parameters can be saved as a template in MinKNOW at the review stage for users who regularly use the same parameters and to save set-up time. The template can be loaded when setting up a new sequencing run but can be reviewed and edited before starting the run.
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Set up a sequencing run on MinKNOW as outlined in the "Starting a sequencing run" section of this protocol.
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On the review tab, click 'Save settings as template' before starting the sequencing run.
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Fill in the template name and click save.
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To use the saved template on a new sequencing run, click 'Start sequencing' and fill in the experiment name and select the flow cell position(s).
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Click 'Load settings from template' and select the template to use. Click load to open the template.
Saved settings can also be deleted from this window by clicking Delete.
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Confirm the kit and flow cell type to load by clicking 'Yes' and fill in the sample ID(s).
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Once the template has loaded, fill in the sample ID(s).
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Review the sequencing parameters on the review tab and start the run by clicking 'Start'.
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Users can also join an existing experiment that has been previously run on the device:
- Fill in the experiment name and select flow cell position.
- Choose the same flow cell type as used in the previous experiment.
- Click Join existing.
- Select the previous experiment to run and click Join.
- The previous experiment parameters will load with the experiment name.
- Fill in the sample ID(s) and review settings in the "Review" tab and start the run.
Note: Flow cell types must be the same between both experiments to join.