February 2023
- Updated legacy wording regarding kit availability in the "Overview of the protocol".
January 2023
- Update the priming and loading the flow cells for PromethION with new diagrams.
Nov 2022
- Updated product phase to Legacy.
August 2022
- Updated product phase to Legacy.
23 June 2022:
- Updated the Adapter ligation and clean-up section to distinguish beteween MinION/GridION preps and PromethION preps, accounting for the difference in volume for the final elution depending on the prep.
- Updated MinION and GridION flow cell loading animations
- Updated compatibilities
23 June 2022:
- Updated the Adapter ligation and clean-up section to destinguish beteween MinION/GridION preps and PromithION preps, accounting for the difference in volume for the final elution depending on the prep.
14th April 2022
Protocol name change
23rd February 2022
- Minor corrections
18th February 2022
- Minor update to volume of nuclease-free water DNA sample is prepared in from 49 µl to the correct 47 µl in the "DNA repair and end-prep" step.
27th January 2022
Updated the "Mixing method" to include pipette mixing 10-20 times for component combination in all ligation reactions, DNA repar and end-prep.
In "Priming and loading the flow cell", added tips/notes for preparing your library and flow cell loading.
Download pdf for Minion Download pdf for Promethion Download pdf for Gridion