07th March 2024
- Updated library loading recommendations to 35-50 fmols.
2nd October 2023
- Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
April 2023
- Added flow cell priming mix volumes to the "Priming and loading" steps
April 2023
- Updated the DNA repair end-prep step mastermix tip.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
January 2023
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
20th October 2022
- EXP-NBA114 information updated with availability
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
4th Oct 2022
- Updated kit 14 info sheet snippet and link