21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
01 May 2024
- Updated snippet in 'Equipment and consumables' to no longer reference SQK-NBD114.96.
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
- Format change in "Native barcode ligation" step to fix downloaded checklist.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
1st August 2023
- Updated recommended magnetic rack
May 2023
- Added amplicon tailed primer sequences to the introduction
April 2023
- Added flow cell priming mix volumes to the "Priming and loading" steps