DRAFT
20th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated protocol to include instructions for mouse samples as well as the original method for human samples.
- Updated software recommendations from Guppy to now use Dorado.
- Updated sequencing and library reload times for optimal pore occupancy and flow cell health.
- Updated downstream analysis instructions to Dorado commands and to include information on nextflow pipelines for wf-human-variation.
- Updated downstream analysis instructions to use modkit bioinformatics tool.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
- Kit updated to released phase and guppy_basecaller parameter updated in "Downstream analysis"
May 2023
- This kit has moved to released phase.
- Updated the guppy_basecaller parameter in "Downstream analysis".
March 2023
- Updated "Data acquisition and basecalling" to correctly reflect basecalling being carried out post-sequencing in the Dpwnstream analysis section of the protocol.
- Added "Ending the experiment" page to the "Sequencing and data analysis" section of the protocol.