21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
4th October 2023
- Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
2nd October 2023
- Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
26th September 2023
- Added duplex information to the Sequencing and data analysis section
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
- Format change in "Native barcode ligation" step to fix downloaded checklist.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
30th June 2023
- Added Flongle branch to the protocol.
April 2023
- Updated the DNA repair end-prep step mastermix tip.
March 2023
- Updated the DNA repair end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
January 2023
- Updated the "Priming and loading the PrometION flow cell" section to the updated version for correct kit reagent vial size.
January 2023
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
20th October 2022
- EXP-NBA114 information updated with availability
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
4th Oct 2022
- Updated kit 14 info sheet snippet and link