19th September 2023
- Updated flow cell loading instructions to include light shield installation
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
17th July 2023
- Updated "washing and reloading a PromethION Flow Cell" to include recommendation to remove waste after 1 hour incubation with nuclease
10th July 2023
- Updated "Data acquisition and basecalling" to use the most recent MinKNOW GUI version in screenshots
- Updated "Downstream analysis" with the most recent recommendations from EPI2ME labs to ensure successful sequencing and data analysis
- Updated recommendation in the "Downstream analysis" to use modkit not modbam2bed has been depricated.
10th July 2023
- Updated "Data acquisition and basecalling" to use the most recent MinKNOW GUI version in screenshots
- Updated "Downstream analysis" with the most recent recommendations from EPI2ME labs to ensure successful sequencing and data analysis
4th July 2023
- Reformated the "Washing and reloading a PromethION Flow Cell" to be consistent with our other flow cell washing protocols and included an extra recommendation to remove waste buffer between priming mix flushes after using the flow cell wash kit.
7th June 2023
- Included a pore scan screenshot in the wash step.
May 2023
- Added flow cell priming mix volumes to the "Priming and loading" steps
- This kit has moved to released phase.
April 2023
- Added flow cell priming mix volumes to the "Priming and loading" steps
February 2023
- Updated checklist to include size selection step.
January 2023
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
10th November 2022
Updated introduction to be inline with the online protocol