February 2023
- Updated product phase to 'Legacy'
06th July 2022
- Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
06th July 2022
- Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
28th January 2022
- Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
23rd Sept 2021
- Updated compatibilities snippet in the 'Overview of the protocol' and updated the flow cell loading recommendations
13th Sept 2021
- Added table outlining kit contents in the Equipment and Consumables section.
9th Dec
Compatibilities updated for EXP-WSH004
21st Feb 2020
Added 'Library storage recommendations' snippet before flow cell loading step
30th September 2019
The optional DNA fragmentation step has been removed from the protocol, since it is now available as a stand-alone protocol.
27th September 2019
Updated and expanded Troubleshooting guide
23rd August 2019
Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
23rd August 2019
Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.