February 2023
- Updated product phase to 'Legacy'
1st August 2022
- Removed references to FFPE in the End-prep step.
- Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
14th July 2022
- Removed references to discontinued flow cells and updated compatibilities
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
October 2021
- Clarified R10.3 flow cell loading recommendations
13th Sept 2021
- Added tables outlining kit contents in the Equipment and consumables section
29th April 2021
- Updated step: end-of-step in the 'End-prep' section to take the end-prepped DNA into the native barcode ligation step.
21st April 2021
Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions:
- EXP-AMII001 is included in the "Equipment and consumables" section
- Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
21st Jan
NEBNext FFPE DNA Repair Mix removed from and DNA repair and end-prep step. This step has also been renamed to 'End-prep'.
9th Dec
Compatibilities updated for EXP-WSH004
14th Sept 2020
Removed references to Flongle from the protocol
21st Feb 2020
Added 'Library storage recommendations' snippet before flow cell loading step
23rd January 2020
Replaced DNA input recommendations for R10 flow cells with R10.3:
- starting with 1.5-3 µg (or 150-300 fmol) DNA
- loading 25-75 fmols onto the flow cell