March 2023
- Updated product phase to discontinued
January 2023
- Update the priming and loading the flow cells for PromethION with new diagrams.
23 Sept 2022
- Updated product phase to legacy
1st August 2022
- Removed references to FFPE in the End-prep step.
- Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
14th April 2022
Protocol name change
24th Febrary 2022
Updated EXP-AUX110 to new name EXP-AUX002
3rd February 2022
- Removed reference to SPRI beads in the Native Barcode Ligation step.
28th January 2022
Updated the equipment and consumables section to include further information on our reagents as well as a minor correction in the consumables section of the Adapter ligation and clean-up step.
27th January 2022
- Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
14th January 2022
In "Priming and loading the flow cell", the first "Important" note has been updated to say that the Kit 12 chemistry now runs at 30°C on all nanopore sequencing devices.