May 2023
- Updated kit availability
February 2023
- Updated product phase to 'Legacy'
9th August 2022
- Added information about the RAP Top-Up kit in the Equipment and Consumables section.
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
13th Sept 2021
- Added table outlining kit contents in the Equipment and Consumables section.
23rd December 2020
Corrected a mistake in naming the Barcode Primers in the PCR Adapters Ligation and Amplification step: LWB 01-12 has been changed to BP 01-12
9th Dec
Compatibilities updated for EXP-WSH004
24th December 2019
- Amended Consumables and Equipment list to include LongAmpĀ® Hot Start Taq 2X Master Mix (NEB, M0533)
- Amended reagent mixing instructions
27th September 2019
Updated and expanded Troubleshooting guide
23rd August 2019
Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
19th July 2019
Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
Updated with new information for MinKNOW release v19.06:
- Compression of queued reads
- Pause functionality
- UI improvements including:
- Flow cell health at platform QC
- Flow cell status on landing page
- R10 flow cell running scripts
- New Fast Models for RNA and DNA
- PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files
- Flongle hardware check for confirming Flongle adapter functions correctly
Update to the flow cell priming section, and instructions for topping up the flow cell with fuel, following the release of the Flow Cell Priming Kit (EXP-FLP002)
Download pdfUpdates for MinKNOW release 19.05
Download pdfUpdates for MinKNOW v18.12.4
Download pdfUpdates for MinKNOW v2.2
Download pdf