May 2023
- Updated kit availability
January 2023
- Changed the PromethION "Priming and loading the flow cell" section to the updated version.
December 2022
- Updated Step 7 in "Selecting for full-length by PCR and barcoding samples" to clarify the example volume of AMPure XP Beads
11th November
- Product status updated to Legacy
25th October 2022
- Added link to newest Kit 11 protocol version
July 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
14th April 2022
Protocol name change
13th Sept 2021
- Kit contents image of the PCR-cDNA Barcoding Sequencing Kit contents (SQK-PCB109) has been updated to reflect the additional Elution Buffer (EB) vials added to the kit. Kit fill volumes have also been added.
- Added table outlining kit contents in the equipment and consumables section.
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
9th Dec
Compatibilites updated for EXP-WSH004