February 2023
- Updated product phase to 'Legacy'
7th July 2022
- A new section has been added to Troubleshooting: "Lot output from PCR"
20th June 2022
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
16th June 2022
"Tiled DNA amplification and clean-up":
- In the primer pooling instructions, "Primer number" has been changed to "Primer pair".
- An additional step has been added: "Immediately before setting up the PCR, dilute each primer pool 1:10 in nuclease-free water to make a 10 μM working stock."
- In the step for setting up the PCR reaction, a new note has been added: "We recommend to make a PCR master mix, either for individual primer pools for multiple samples or one for the three primers sets."
"DNA repair and end-prep":
- After resuspending the bead pellet in 25 µl nuclease-free water, the incubation time has been extended from 2 minutes to 5 minutes.
"Adapter ligation and clean-up":
- The AMPure XP bead purification step has been changed from 0.5X beads to 0.4X beads (40 μl of AMPure XP beads added to the reaction).