19th September 2023
- Updated flow cell loading and washing instructions to include light shield installation
17th July 2023
- Updated recommendations for running a second library on a PromethION Flow Cell straigh away and added a step to remove waste after 1 hour incubation during nuclease wash.
April 2023
- Updated flow cell check recommendations after storing a flow cell
March 2023
- Updated the protocol title from 'Running multiple PromethION flow cells' to 'Loading multiple PromethION Flow Cells'
- Updated the introduction to the protocol
- Updated the equipment and consumables for Kit 14 chemistry
- Updated the flow cell loading animations and included a new video illustrating the entire new process
- Included excess volumes required for scaling up priming mix volumes
- Updated the screenshot of what users can expect to see on MinKNOW when washing and reusing a flow cells
March 2023
- Updated the protocol title from 'Running multiple PromethION flow cells' to 'Loading multiple PromethION Flow Cells'
- Updated the introduction to the protocol
- Updated the equipment and consumables for Kit 14 chemistry
- Updated the flow cell loading animations and included a new video illustrating the entire new process
- Included excess volumes required for scaling up priming mix volumes
- Updated the screenshot of what users can expect to see on MinKNOW when washing and reusing a flow cells
24th Febrary 2022
Updated EXP-AUX110 to new name EXP-AUX002
22nd July 2021
In the "Washing multiple flow cells on a PromethION" section, the instructions for washing the flow cells have been updated with the correct volumes of Wash Mix and Wash Diluent:
- For 24 flow cells: Add 48 µl of thawed and mixed Wash Mix (WMX) to 9.552 ml of thawed and mixed Wash Diluent (DIL)
- For 48 flow cells: Add 96 µl of thawed and mixed Wash Mix (WMX) to 19.104 ml of thawed and mixed Wash Diluent (DIL)
10th June 2021
In the "Priming and loading multiple flow cells on a PromethION" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
28th January 2021
- Updated materials and instructions for the Flow Cell Wash Kit from EXP-WSH003 to EXP-WSH004
23/11/20
Updated scaled volumes for flush tether and buffer
23/11/20
Updated scaled volumes for flush tether and buffer
19/8/20
Updated scaled volumes + recommended flush tether and buffer at room temp