21st August 2024
- Added warning to the "Post-pull-down PCR" step to ensure users DO NOT allow the amplicon-bead conjugate to pellet before incubating.
- NEB reagent recommendations for purchase and use updated (Salt T4 ligase in place of Quick Ligation Module).
- Updated the Adapter ligation step with new recommended method.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
30 July 2024
- Updated wording in protocol introduction and in equipment and consumables: "This protocol is compatible and fully supported with 10X Genomics Next GEM Single Cell 3' Kit (V3.1) and the Visium Spatial Gene Expression Kit (V1). Other versions of the kits are not supported."
30 July 2024
- Updated wording in protocol introduction and in equipment and consumables: "This protocol is compatible and fully supported with 10X Genomics Next GEM Single Cell 3' Kit (V3.1) and the Visium Spatial Gene Expression Kit (V1). Other versions of the kits are not supported."
07 May 2024
- Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell.
07 May 2024
- Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell.
February 2024
- Udated protocol to be specific to 3' cDNA (New 5' cDNA specific 10X protocol now also available on the community).