- Materials
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- Rapid Sequencing Kit V14 (SQK-RAD114)
- Control Expansion (EXP-CTL001)
- Consumables
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- 1.5 ml Eppendorf DNA LoBind tubes
- 0.2 ml thin-walled PCR tubes
- Nuclease-free water (e.g. ThermoFisher, AM9937)
- Bovine Serum Albumin (BSA) (50 mg/ml) (e.g Invitrogen™ UltraPure™ BSA 50 mg/ml, AM2616)
- Equipment
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- Microfuge
- P1000 pipette and tips
- P100 pipette and tips
- P20 pipette and tips
- P10 pipette and tips
- P2 pipette and tips
- Timer
- Thermal cycler or heat blocks
- Optional equipment
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- Qubit fluorometer (or equivalent for QC check)
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For this protocol, you will need 100 ng Lambda DNA (LMD) supplied in the Control Expansion (EXP-CTL001) pack.
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Rapid Sequencing Kit V14 (SQK-RAD114) contents
Note: We are in the process of reformatting our kits with single-use tubes into a bottle format.
Single-use tubes format:
Bottle format:
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Control Expansion (EXP-CTL001) contents
Note: The DNA Control Sample (DCS) is a 3.6 kb standard amplicon mapping the 3' end of the Lambda genome. Lambda DNA (LMD) fragment length is 48.5 kb.