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Rapid Sequencing Kit features
This kit is recommended for users who:
- require a short preparation time
- have limited starting input
- have limited access to laboratory equipment
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Introduction to Rapid Lambda Control protocol (SQK-RAD114)
This protocol describes the step-by-step instructions to complete a Lambda Control experiment using the Rapid Sequencing Kit V14 (SQK-RAD114) on a MinION flow cell. The control experiment uses a high-quality DNA sample supplied in the kit. It allows you to become familiar with nanopore sequencing before preparing your own library.
Steps in the sequencing workflow:
Prepare for your experiment
You will need to:
- Ensure you have your sequencing kit, the correct equipment and third-party reagents
- Download the software for acquiring and analysing your data
- Check your flow cell to ensure it has enough pores for a good sequencing run
Library preparation
You will need to:
- Tagment your DNA using the Fragmentation Mix in the kit
- Attach sequencing adapters supplied in the kit to the DNA ends
- Prime the flow cell, and load your DNA library into the flow cell
Sequencing and analysis
You will need to:
- Start a sequencing run using the MinKNOW software, which will collect raw data from the device and convert it into basecalled reads
- Optional: Start the EPI2ME software and select a workflow for further analysis, e.g. metagenomic analysis or drug resistance mapping