Your MinION Mk1B and flow cell should still be connected. Check your MinION Mk1B is plugged in to your computer's USB 3.0 port, and the flow cell is clipped firmly into the MinION Mk1B device.

Check the MinKNOW software is still open. If you have closed it, double-click the icon on the desktop and login.

Select Start Sequencing on the home page.

On the New experiment popup screen, select the running parameters for your experiment from the individual tabs.

Select positions
The postions tab will show the chosen flow cell. An experiment name and sample ID can then be assigned.
The other tabs will not become available until an experiment name has been provided.

Kit selection
The kit selection tab will provide a dropdown of available kits. Select SQK-LSK114.

A pop-up will appear prompting you to choose the sequencing speed. Select the Default (400 bps) option.

Click Skip to final review.

On the final page, there will be a summary of all the options chosen for the experiment.
Click Start.

When sequencing is initiated, the software will move to the System Overview. The flow cell will cycle through all 2048 channels to select the best set of 512 to start the run.

Once the best combination of channels is selected, sequencing will begin on those channels automatically.

You will be indicated sequencing has started when flow cell health is displayed and a progress bar will appear beneath the flow cell.