Guide - MinION - Gsl_9165_v114_revb_13jul2022- 17/32

5

Sequencing run

Approx 40 Mins

Goal: to prime your flow cell with buffer, load your DNA library into the flow cell, and start a sequencing experiment in MinKNOW.

This step will take you through flow cell priming, sample loading and starting your sequencing experiment.

What you will need

1.5 ml Eppendorf DNA LoBind tubes
Bovine Serum Albumin (BSA) (50 mg/ml) (e.g Invitrogen™ UltraPure™ BSA 50 mg/ml, cat# AM2616)
Flow Cell Flush (FCF)
Flow Cell Tether (FCT)
Lambda DNA library (Tube 1)
Library Beads (LIB)
Microfuge
P100 pipette and tips
P100 pipettes and tips
P1000 pipette and tips
P20 pipettes and tips
Sequencing Buffer (SB)

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