The following steps will introduce you to flow cell fluidics and handling.

Flow cells are shipped with storage buffer to maintain product integrity. The priming step flushes out the storage buffer and replaces it with a mix of Flow Cell Flush (FCF) and Flow Cell Tether (FCT). The DNA library is then loaded into the flow cell.

Throughout the process it is essential that the sensor array remains submerged in buffer at all times. If an air bubble passes over any channels, those pores will be permanently damaged. It is therefore important to avoid introducing air in the pipette tips, and remove any air bubbles in the flow cell ports.

Priming and loading sample into flow cells is best done with minimal movement. For this reason, please do all subsequent steps on a MinION Mk1B device on a flat surface.

The video below highlights good practices for priming and loading the flow cell: