Make sure the contents of the Flow Cell Flush (FCF) and Flow Cell Tether (FCT) tubes are fully defrosted. Mix the tubes by pipetting up and down, and then spin down in a microfuge.

Transfer 30 µl of Flow Cell Tether (FCT) directly into one of the Flow Cell Flush (FCF) tubes.

Add 5 µl of Bovine Serum Albumin (BSA) at 50 mg/ml to the tube of Flow Cell Flush (FCF).

Mix the contents of the tube by flicking the tube with your finger and then spin down briefly in a microfuge.