The pre-sequencing mix is a mix of your RNA library, water, and RNA Running Buffer (RRB) that is loaded into the flow cell.

Please ensure you have completed the Flow Cell Check section before preparing your pre-sequencing mix.

Take the 1.5 ml tube containing your control RNA library (Tube 5), and add 17.5 µl nuclease-free water.

Add 37.5 µl RNA Running Buffer (RRB) to the tube.

• Mix the contents of the tube thoroughly by flicking the tube with your finger
• Spin down briefly in a microfuge