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5. Sequencing run
  • Introduction
  • 1. Software installation
  • 2. Hardware check
  • 3. Flow cell check
  • 4. Library preparation
  • 5. Sequencing run
  • 6. Results and metrics
  • 7. Next steps
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You are in the ‘Windows’ branch of Software installation.
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5.2

Prepare your priming mix

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Make sure the contents of the Flush Buffer (FB) and Flush Tether (FLT) tubes are fully defrosted. Mix the tubes by pipetting up and down, and then spin down in a microfuge.

Transfer 30 µl of Flush Tether (FLT) directly into one of the Flush Buffer (FB) tubes.

Priming-Mix

Mix the contents of the tube by flicking the tube with your finger and then spin down briefly in a microfuge.

Priming mix

The resulting buffer is now referred to as the Priming Mix (FB tube). You will use this to flush out the storage buffer from the flow cell.

Prev 5.3 Prime your flow cell

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