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Ligation Sequencing Kit features
This kit is recommended for users who:
- Would like to achieve raw read sequencing modal accuracy of Q20+ (99%) or above
- Require control over read length
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Introduction to the Ligation Sequencing Kit (SQK-LSK112) protocol
This protocol describes how to carry out sequencing of amplicon sample using the Ligation Sequencing Kit (SQK-LSK112). It is recommended that a Lambda control experiment is completed first to become familiar with the technology.
Steps in the sequencing workflow:
Prepare for your experiment
You will need to:
- Extract your DNA, and check its length, quantity and purity. The quality checks performed during the protocol are essential in ensuring experimental success.
- Ensure you have your sequencing kit, the correct equipment and third-party reagents
- Download the software for acquiring and analysing your data
- Check your flow cell to ensure it has enough pores for a good sequencing run
Library preparationYou will need to:
- Repair the DNA, and prepare the DNA ends for adapter attachment
- Attach sequencing adapters supplied in the kit to the DNA ends
- Prime the flow cell, and load your DNA library into the flow cell
Sequencing and analysis
You will need to:
- Start a sequencing run using the MinKNOW software which will collect raw data and convert it into basecalled reads for R10.4 flow cells. For other flow cell types, basecalling is done offline at the end of the sequencing run.