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Field Sequencing Kit features
Use this kit if you:
- Need a fast library prep time (10 mins)
- Have limited laboratory experience
- Are sequencing in the field, with limited access to laboratory equipment
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Introduction to Field Sequencing Kit protocol (SQK-LRK001)
This protocol describes the step-by-step instructions to complete a rapid sequencing of genomic DNA using the Field Sequencing Kit (SQK-LRK001).
Steps in the sequencing workflow:
Prepare for your experiment
You will need to:
- Extract your DNA, and check its length, quantity and purity.
The quality checks performed during the protocol are recommended for ensuring experimental success.
- Ensure you have your sequencing kit, and the correct equipment
- If not already installed, download the software for acquiring and analysing your data
- Check your flow cell(s) to ensure it has enough pores for a good sequencing runLibrary preparation
You will need to:
- Tagment your DNA using the Fragmentation Mix in the kit
- Attach sequencing adapters supplied in the kit to the DNA ends
- Prime the flow cell, and load your DNA library into the flow cellSequencing and analysis
You will need to:
- Start a sequencing run using the MinKNOW software, which will collect raw data from the device and convert it into basecalled reads
- optional Start the EPI2ME software and select a workflow for further analysis, e.g. metagenomic analysis or drug resistance mapping