- Consumables
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- PromethION Flow Cell
- Equipment
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- PromethION 24/48 device
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How it works
Flow cells are shipped with a QC DNA molecule present in the buffer. This molecule produces a distinctive nanopore signal. The PromethION software uses this signal to validate the integrity of the nanopore array before use and provides the user with an estimate of the number of simultaneously available channels for the experiment. Active pores are reported in four groups, each of which may be used in turn when running long experiments e.g. a 64 hour sequencing run. Shorter experiments may not use all groups.
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Load the flow cells to be run into the docking ports of the PromethION.
Insert the chip connector fully into the docking port. Take note to ensure the latch to hold the chip in place clicks in.
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Double-click the MinKNOW icon located on the desktop to open the MinKNOW UI.
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We recommend users to log into the MinKNOW software using their Community credentials.
To log in, you must be connected to the internet.
From here, you can also change the language in the user interface in the bottom right corner.
You will be prompted to enter your email address and Nanopore password:
If you experience login issues, please visit the Community Support channel (https://community.nanoporetech.com/support) and use Continue as guest for temporary use.
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Click the sequencing device connected to the computer.
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The Sequencing Overview page should show the flow cell has not had any checks carried out.
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When you see the flow cell type and flow cell IDs recognised, click Start to begin.
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The flow cell check should take a few minutes.
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Flow cell health indicators
The quality of the flow cell will be shown as one of the three outcomes:
Yellow exclamation mark (Flongle flow cell): The number of sequencing pores is below warranty. Take the flow cell out of the device, re-insert it and run a flow cell check again. If the flow cell is still below warranty, contact support@nanoporetech.com
Green tick (MinION flow cell): The number of sequencing pores is above warranty.
Question mark (PromethION flow cell): A flow cell check has not been run on the flow cell during this MinKNOW session.
Note: The indicator of quality will only remain visible during a MinKNOW session. Once the MinKNOW session has ended, the status of the flow cell will be erased.