- Materials
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- Flow Cell Wash Kit (EXP-WSH003)
- Optional equipment
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- P1000 pipette and tips
- P20 pipette and tips
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Thaw one tube of Storage Buffer (S) at room temperature.
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Mix contents thoroughly by pipetting and spin down briefly.
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Slide the flow cell priming port cover clockwise to open.
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After opening the priming port, check for a small air bubble under the cover. Draw back a small volume to remove any bubbles:
- Set a P1000 pipette to 200 µl.
- Insert the tip into the flow cell priming port.
- Turn the wheel until the dial shows 220-230 µl, or until you can see a small volume of buffer/liquid entering the pipette tip.
- Visually check that there is continuous buffer from the flow cell priming port across the sensor array.
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Slowly add 500 μl of Storage Buffer (S) through the flow cell priming port.
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Close the priming port.
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Remove all fluid from the waste channel through waste port 1 using a P1000 pipette.
As both the flow cell priming port and SpotON sample port are closed, no fluid should leave the sensor array area.
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The flow cell can now be stored at 4-8°C.