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Ligation Sequencing Kit features
This kit is recommended for users who:
- Would like to achieve raw read sequencing modal accuracy of Q20+ (99%) or above
- Require control over read length
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Introduction to the Lambda Control Experiment (SQK-LSK112) protocol
This protocol describes how to carry out sequencing of a Lambda control DNA sample using the Ligation Sequencing Kit (SQK-LSK112) and the Control Expansion (EXP-CTL001).
Steps in the sequencing workflow:
Prepare for your experiment
You will need to:
- Ensure you have your sequencing kit, the correct equipment and third-party reagents
- Download the software for acquiring and analysing your data
- Check your flow cell to ensure it has enough pores for a good sequencing run
Library preparationYou will need to:
- Repair the DNA, and prepare the DNA ends for adapter attachment
- Attach sequencing adapters supplied in the kit to the DNA ends
- Prime the flow cell, and load your DNA library into the flow cell
Sequencing and analysis
You will need to:
- Start a sequencing run using the MinKNOW software, selecting "SQK-LSK112 - control experiment" in kit selection. Note: When using the control experiment kit, run length will automatically be reduced to 6 hours.
- Align the basecalled data to the Lambda genome using the EPI2ME software.