Overview
- We advise all customers to read and consider the information in the Kit 14 sequencing and duplex basecalling info sheet before starting this protocol
- Requires the PCR Expansion (EXP-PCA001) with the Ligation Sequencing Kit V14 (SQK-LSK114)
- This protocol uses genomic DNA, amplicons or cDNA
- High yield
- Library preparation time ~140 minutes + PCR
- Includes PCR steps
- Compatible with R10.4.1 flow cells
For Research Use Only
Introduction to the protocol
Library preparation
Sequencing and data analysis
Troubleshooting