Overview
This protocol:
- requires the PCR Expansion (EXP-PCA001) with the Ligation Sequencing Kit V14 (SQK-LSK114)
- uses genomic DNA, amplicons or cDNA
- results in high yield
- has a library preparation time ~140 minutes + PCR
- includes PCR steps
- is compatible with R10.4.1 flow cells
For Research Use Only
Introduction to the protocol
Library preparation
Sequencing and data analysis
Troubleshooting