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DNA extraction
Extract DNA from your sample using one of our recommended extraction protocols. For the benchmarking of this method, the Oxford Nanopore team extracted DNA from ~5 million cells using the protocol: Human cell line DNA – QIAGEN Puregene Cell Kit. Please note, this method is also suitable for mouse cell line DNA.
We also offer multiple mammalian sample extraction protocols, which you can use for other sample types.
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DNA fragmentation
The DNA was sheared using the protocol for Covaris g-TUBE fragmentation, with the following modifications:
- The input was 2 μg of DNA in 50 μl.
- The DNA was fragmented in the g-TUBE at 11,000 rpm in 30 sec pulses. The resulting fragment length should be ~6-7 kb.
Take forward 48 μl of your fragmented DNA into the next step.